An iridoid, lamiide, its extraction method from <em>Phlomis bruguieri</em>, and evaluation of its antioxidant capacity

Phlomis bruguieri is a native plant in Iran, which deserves phytochemical study. The aim of this study was evaluation its iridoid contents along with antioxidant capacity. Aerial parts the material were extracted methanol and applied on repeated normal column chromatography using hexane: chloroform (70:30), chloroform: (90:10), (100%). fraction eluted (100%) containing polar glycosides selected medium pressure liquid (MPLC) RP-18 cartridge. Fraction elute by MeOH: water 30:70 loaded HPLC for final purification. Structure elucidation done 1D 2D NMR, mass spectra. Antioxidant activity including total capacity, DPPH FRAP methods designated to assess vitro capacities. This indicated presence lamiide as compound aerial first time [350 mg/2 kg; 0.0175 % (w/w)]. Lamiide showed moderate TAC (EC50: 55 μg/mL; ascorbic acid EC50: 7.5 μg/mL), 116.2 ± 3.51 acid:34.7 0.97 (EC50:&gt;200 quercetin: 0.68 μg/mL) methods.